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Coding

Part:BBa_K1315000:Design

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-20)


pqsR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 461
    Illegal BamHI site found at 77
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 645


Design Notes

The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.


Source

The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.

References